In Vitro ADME

In Vitro ADME Screening

In vitro screening is a critical component in identifying candidate compounds for  clinical development. In Vitro assays serve as surrogates and indicators to better predict absorption, distribution, metabolism and excretion of a compound in vivo and assess potential drug-drug interactions.

BRC provides cost-effective solutions for both standard and custom in vitro protocols with high quality and reproducible results in a short lead time.

Our Offerings

  • Physiochemical Screening

    • Aqueous Solubility Assay
      • Kinetic
      • Thermodynamic
    • Lipophilicity (Log D and Log P)
      • Octanol-water/phosphate buffer partitioning

  • Absorption / Permeability

    • Parallel Artificial Membrane Permeability Assay (PAMPA)
      • PAMPA – GIT
      • PAMPA - BBB

  • Distribution

    • Plasma Protein Binding Assay (Equilibrium Dialysis)
      • Human
      • Rodent: Rat and Mice
      • Non-Rodent: Beagle dog
    • Tissue Binding Assay (Equilibrium Dialysis)
      • Rodent: Rat and Mice
    • Blood Plasma Partitioning Assay 
      • Human
      • Rodent: Rat and Mice
      • Non-Rodent: Beagle dog
    • Microsomal Binding Assay (Equilibrium Dialysis)
      • Human Liver Microsomes
      • Rodent Liver Microsomes: Rat and Mice
      • Non-Rodent Liver Microsomes: Beagle dog

  • Metabolism

    • Matrix Stability Assay (Matrix & Buffer)
      • Ex vivo matrices (Blood, Plasma, Serum, Tissue, Homogenates, CSF)
      • Aqueous Buffers
    • Metabolic Stability 
      • Liver Microsomes
      • S9 fractions
      • Pooled Cryopreserved Hepatocytes
      • Human
      • Rodent: Rat and Mice
      • Non-Rodent: Beagle dog

  • Drug Interaction Studies

    • CYP450 Inhibition
      • Pooled Human Liver Microsomes (HLM)
      • Major Isozymes: CYP1A2, CYP2A6, CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2D6, CYP2E1 and CYP3A4/5
    • CYP450 Time Dependent Inhibition
      • Pooled Human Liver Microsomes (HLM)
      • Major Isozymes: CYP1A2, CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2D6, and CYP3A4
    • CYP450 Reaction Phenotyping
      • Pooled Human Liver Microsomes (HLM)
      • CYP isoforms: CYP1A2, CYP2B6, CYP2C8, CYP2C9, CYP2D6 and CYP3A4
      • Isoform-specific chemical inhibitors and recombinant enzymes

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